Back to Search
Start Over
Picroside II attenuates ischemia/reperfusion testicular injury by alleviating oxidative stress and apoptosis through reducing nitric oxide synthesis
- Source :
- Acta Cirúrgica Brasileira, Vol 34, Iss 11 (2019)
- Publication Year :
- 2019
- Publisher :
- Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2019.
-
Abstract
- Abstract Purpose: To investigate the effect of Picroside II on testicular ischemia and reperfusion (l/R) injury and the underlying mechanism. Methods: Sprague-Dawley rats were randomly divided into 4 groups: sham operated group (Sham), Sham with Picroside II treatment group (Sham+ Pic II), l/R group (l/R) and l/R with Picroside II treatment group (I/R+ Pic II). l/R model was established by rotating the left testis 720° in a clock-wise direction for 4 hours. The histopathologic and spermatogenetic evaluation was performed. The apoptosis changes and the levels of HO-1 (heme oxygenase-1), MPO (myeloperoxidase), NOX (NADPH oxidase), SOD (superoxide dismutase), XO (xanthine oxidase) and NOS (nitric oxide synthase) were measured. Results: The seminiferous tubules were damaged in l/R rats, but Picroside II alleviated the changes induced by l/R. The increased level of apoptosis was decreased by Picroside II (P=0.01, 9.05±0.35 vs. 4.85±0.25). The activities of HO-1, MPO, NOX, XO and MDA content were increased and the SOD activity was decreased in l/R (P
- Subjects :
- Picroside
Ischemia
Reperfusion
Oxidative Stress
Apoptosis
Testis
Rats
Surgery
RD1-811
Subjects
Details
- Language :
- English
- ISSN :
- 01028650
- Volume :
- 34
- Issue :
- 11
- Database :
- Directory of Open Access Journals
- Journal :
- Acta Cirúrgica Brasileira
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4bc88f374eb45e78e92afbb28c4a5b9
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/s0102-865020190110000002