Back to Search
Start Over
Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes
- Source :
- Pifu-xingbing zhenliaoxue zazhi, Vol 28, Iss 3, Pp 182-185 (2021)
- Publication Year :
- 2021
- Publisher :
- editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2021.
-
Abstract
- Objective: To investigate the effect of indirubin on the expression of proinflammatory cytokines in HaCaT cells, and to elucidate the mechanisms of Diqing ointment in the treatment of psoriasis. Methods: The human immortalized keratinocyte cell line, HaCaT cells, were used to observe the changes in the expression levels of IL-1β, IL-6, and TNF-α in HaCaT cells after treatment with different concentrations of indirubin. HaCaT cells were divided into 4 groups. The secretion levels of IL-1β, IL-6, and TNF-α in the culture supernatants were detected by enzyme-linked immunosorbent assay ELISA, and the expression levels of IL-1β, IL-6, and TNF-α mRNA were detected by real-time PCR (RT-qPCR). Results: Expression levels of both mRNA and protein of IL-1β, IL-6, and TNF-α were significantly and dose-dependently decreased after treatment with indirubin. Conclusion: Indirubin, the main component of Qingdai ointment, can lower expression levels of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in keratinocytes, leading to the improvements of psoriasis.
- Subjects :
- indirubin
psoriasis
hacat cell
il-1β
il-6
tnf-α
Dermatology
RL1-803
Subjects
Details
- Language :
- Chinese
- ISSN :
- 16748468
- Volume :
- 28
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Pifu-xingbing zhenliaoxue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4c4a0c608bf04716ae7f19eb5716fad2
- Document Type :
- article
- Full Text :
- https://doi.org/10.3969/j.issn.1674-8468.2021.03.005