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Characterization and Mapping of a Rolling Leaf Mutant Allele rlT73 on Chromosome 1BL of Wheat

Authors :
Lin Huang
Meijuan Gan
Wenzhuo Zhao
Yanling Hu
Lilin Du
Yuqin Li
Kanghui Zeng
Dandan Wu
Ming Hao
Shunzong Ning
Zhongwei Yuan
Lihua Feng
Lianquan Zhang
Bihua Wu
Dengcai Liu
Source :
International Journal of Molecular Sciences, Vol 25, Iss 7, p 4103 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Leaf rolling is regarded as an important morphological trait in wheat breeding. Moderate leaf rolling is helpful to keep leaves upright and improve the photosynthesis of plants, leading to increased yield. However, studies on the identification of genomic regions/genes associated with rolling leaf have been reported less frequently in wheat. In this study, a rolling leaf mutant, T73, which has paired spikelets, dwarfism, and delayed heading traits, was obtained from a common wheat landrace through ethyl methanesulfonate mutagenesis. The rlT73 mutation caused an increase in the number of epidermal cells on the abaxial side and the shrinkage of bulliform cells on the adaxial side, leading to an adaxially rolling leaf phenotype. Genetic analysis showed that the rolling leaf phenotype was controlled by a single recessive gene. Further Wheat55K single nucleotide polymorphism array-based bulked segregant analysis and molecular marker mapping delimited rlT73 to a physical interval of 300.29–318.33 Mb on the chromosome arm 1BL in the Chinese Spring genome. We show that a point mutation at the miRNA165/166 binding site of the HD zipper class III transcription factor on 1BL altered its transcriptional level, which may be responsible for the rolling leaf phenotype. Our results suggest the important role of rlT73 in regulating wheat leaf development and the potential of miRNA-based gene regulation for crop trait improvement.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
7
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.4c8d830a81c44f168a1f36742a218385
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25074103