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Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays
- Source :
- Transplantation Direct, Vol 3, Iss 11, p e218 (2017)
- Publication Year :
- 2017
- Publisher :
- Wolters Kluwer, 2017.
-
Abstract
- Background. Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms. Methods. Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients. Results. Most HLA class I (94.5%) and HLA class II (89%) Abs with moderate to high MFI titer (≥4000) were detected by both assays. A modest correlation was observed between MFI values obtained from the 2 assays for both class I (r = 0.3, r2 = 0.09, P < 0.0001) and class II Ab (r = 0.707, r2 = 0.5, P < 0.0001). Both assays detected anti–class I and II Ab that the other did not; however, no specific HLA allele was detected preferentially by either of the 2 assays. For a limited number of discrepant sera, dilution resulted in comparable reactivity profiles between the 2 platforms. Conclusions. Immucor and One Lambda/ThermoFisher assays have a similar, albeit nonidentical, ability to detect anti-HLA Ab. Although the correlation between the assays was present, significant variances exist, some of which can be explained by a dilution-sensitive “prozone” effect.
Details
- Language :
- English
- ISSN :
- 23738731 and 00000000
- Volume :
- 3
- Issue :
- 11
- Database :
- Directory of Open Access Journals
- Journal :
- Transplantation Direct
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4cb29c8a14459d851c45b888b1e31b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1097/TXD.0000000000000734