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Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

Authors :
Hyeon-Jeong Go
Byung-Joo Park
Hee-Seop Ahn
Dong-Hwi Kim
Da-Yoon Kim
Jae-Hyeong Kim
Joong-Bok Lee
Seung-Yong Park
Chang-Seon Song
Sang-Won Lee
Yang-Kyu Choi
In-Soo Choi
Source :
Vaccines, Vol 9, Iss 11, p 1265 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 μg VLP-, and 200 μg VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 106 HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 μg VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 μg VLP-vaccinated pigs. The 100 and 200 μg VLP-vaccinated pigs had significantly higher (p < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6–10 with normal levels of liver enzymes. The 200 μg VLP-vaccinated pigs showed statistically less liver tissue fibrosis (p < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.

Details

Language :
English
ISSN :
2076393X
Volume :
9
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Vaccines
Publication Type :
Academic Journal
Accession number :
edsdoj.4d64f34af7ef44a28d19c52dc6ea7ff8
Document Type :
article
Full Text :
https://doi.org/10.3390/vaccines9111265