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Study on Optimization and Activities of Rapeseed Meal Protein by Alkali-extraction and Acid-precipitation
- Source :
- Shipin gongye ke-ji, Vol 43, Iss 9, Pp 164-171 (2022)
- Publication Year :
- 2022
- Publisher :
- The editorial department of Science and Technology of Food Industry, 2022.
-
Abstract
- The aim of the study was to improve the yield of rapeseed meal protein and reduce the content of glucosinolate by optimizing the alkali-extraction and acid-precipitation process, and the bioactivities were also explored. And single factor experiment and response surface analysis were used. The results showed that the optimal conditions were as follows, solid-liquid ratio 1:40 g/mL, pH13.1, temperature 46 ℃ and extraction time 82 min. Under these conditions, the average yield of protein and glucosinolate content were 8.78% and 1.65%. And then pH4.0 was used for protein precipitation, while the sediments and supernatant were processed by vacuum freeze-drying and spray-drying respectively and correspondingly two sorts of protein extractions, rapeseed meal precipitated protein and rapeseed meal acid soluble protein. Furthermore, SDS-PAGE analysis showed that their main components were cruciferin (Mw 31 kDa、14~22 kDa) and napin (Mw
Details
- Language :
- Chinese
- ISSN :
- 10020306
- Volume :
- 43
- Issue :
- 9
- Database :
- Directory of Open Access Journals
- Journal :
- Shipin gongye ke-ji
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4db0009e61406f849c5f2ee1a95f8c
- Document Type :
- article
- Full Text :
- https://doi.org/10.13386/j.issn1002-0306.2021080100