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Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells
- Source :
- Journal of Integrative Agriculture, Vol 17, Iss 5, Pp 1171-1180 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication. In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific. Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10. Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production. Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro.
Details
- Language :
- English
- ISSN :
- 20953119
- Volume :
- 17
- Issue :
- 5
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Integrative Agriculture
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.4e074e3f2774f29b55795e94652b935
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/S2095-3119(17)61896-3