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Novel biomarker combination improves the diagnosis of serious bacterial infections in Malawian children

Authors :
Irwin Adam D
Marriage Fiona
Mankhambo Limangeni A
Group IPD
Jeffers Graham
Kolamunnage-Dona Ruwanthi
Guiver Malcolm
Denis Brigitte
Molyneux Elizabeth M
Molyneux Malcolm E
Day Philip J
Carrol Enitan D
Source :
BMC Medical Genomics, Vol 5, Iss 1, p 13 (2012)
Publication Year :
2012
Publisher :
BMC, 2012.

Abstract

Abstract Background High throughput technologies offer insight into disease processes and heightens opportunities for improved diagnostics. Using transcriptomic analyses, we aimed to discover and to evaluate the clinical validity of a combination of reliable and functionally important biomarkers of serious bacterial infection (SBI). Methods We identified three previously reported biomarkers of infection (neutrophil gelatinase-associated lipocalin (NGAL), granulysin and resistin) and measured gene expression using quantitative real-time PCR. Protein products related to the three transcripts were measured by immunoassays. Results Relative gene expression values of NGAL and resistin were significantly increased, and expression of granulysin significantly decreased in cases compared to controls. Plasma concentrations of NGAL and resistin were significantly increased in children with confirmed SBI compared to children with no detectable bacterial infection (NBI), and to controls (287 versus 128 versus 62 ng/ml and 195 versus 90 versus 18 ng/ml, respectively, p Conclusions We have demonstrated a unique combination of diagnostic biomarkers of SBI using transcriptomics, and demonstrated translational concordance with the corresponding protein. The addition of NGAL and resistin protein measurement to procalcitonin significantly improved the diagnosis of SBI.

Details

Language :
English
ISSN :
17558794
Volume :
5
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Medical Genomics
Publication Type :
Academic Journal
Accession number :
edsdoj.4eaea8c4539445729bc8151111bc2e1a
Document Type :
article
Full Text :
https://doi.org/10.1186/1755-8794-5-13