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scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells

Authors :
Stephen J. Clark
Ricard Argelaguet
Chantriolnt-Andreas Kapourani
Thomas M. Stubbs
Heather J. Lee
Celia Alda-Catalinas
Felix Krueger
Guido Sanguinetti
Gavin Kelsey
John C. Marioni
Oliver Stegle
Wolf Reik
Source :
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Publication Year :
2018
Publisher :
Nature Portfolio, 2018.

Abstract

Relationships between DNA methylation and transcription, and methylation and DNA accessibility can be probed but interrogating all three in the same single cells has not been possible. Here, the authors report the first single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.5160af085446eb92ec31544e77c471
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-018-03149-4