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UvrY is required for the full virulence of Aeromonas dhakensis

Authors :
Yi-Wei Chen
Wen-Hsuan Yeh
Hung-Jen Tang
Jenn-Wei Chen
Hung-Yu Shu
Yu-Chen Su
Sin-Tian Wang
Cheng-Ju Kuo
Yin-Ching Chuang
Chi-Chung Chen
Wen-Chien Ko
Chang-Shi Chen
Po-Lin Chen
Source :
Virulence, Vol 11, Iss 1, Pp 502-520 (2020)
Publication Year :
2020
Publisher :
Taylor & Francis Group, 2020.

Abstract

Aeromonas dhakensis is an emerging human pathogen which causes fast and severe infections worldwide. Under the gradual pressure of lacking useful antibiotics, finding a new strategy against A. dhakensis infection is urgent. To understand its pathogenesis, we created an A. dhakensis AAK1 mini-Tn10 transposon library to study the mechanism of A. dhakensis infection. By using a Caenorhabditis elegans model, we established a screening platform for the purpose of identifying attenuated mutants. The uvrY mutant, which conferred the most attenuated toxicity toward C. elegans, was identified. The uvrY mutant was also less virulent in C2C12 fibroblast and mice models, in line with in vitro results. To further elucidate the mechanism of UvrY in controlling the toxicity in A. dhakensis, we conducted a transcriptomic analysis. The RNAseq results showed that the expression of a unique hemolysin ahh1 and other virulence factors were regulated by UvrY. Complementation of Ahh1, one of the most important virulence factors, rescued the pore-formation phenotype of uvrY mutant in C. elegans; however, complementation of ahh1 endogenous promoter-driven ahh1 could not produce Ahh1 and rescue the virulence in the uvrY mutant. These findings suggest that UvrY is required for the expression of Ahh1 in A. dhakensis. Taken together, our results suggested that UvrY controls several different virulence factors and is required for the full virulence of A. dhakensis. The two-component regulator UvrY therefore a potential therapeutic target which is worthy of further study.

Details

Language :
English
ISSN :
21505594 and 21505608
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Virulence
Publication Type :
Academic Journal
Accession number :
edsdoj.530cc0ee246f45fe84486ff8979bdc5d
Document Type :
article
Full Text :
https://doi.org/10.1080/21505594.2020.1768339