Electrospun composites of poly-3-hydroxybutyrate reinforced with conductive fillers for in vivo bone regeneration

The aim of this study was to investigate the biocompatibility and osteoinductive properties of fibrous scaffolds implanted in the bone tissue of white rats in vivo. The effect of various concentrations of conductive PANi (2 and 3 wt%) and rGO (0.2 and 1 wt%) in the PHB scaffolds on the reparative processes of bone callus formation in white rats was studied. On the 28th day after implantation, a histomorphological study of the preparations of the transverse section of the diaphysis of the femur of the implantation area was performed. The results of implantation tests in the femur revealed that all the studied scaffolds are biocompatible and the most pronounced stimulating effect on bone formation was observed for hybrid PHB scaffolds doped with 3 wt% PANi and 0.2 wt% rGO compared with pure PHB scaffolds, thus determining further prospects to study the osteoinductive features of hybrid PHB scaffolds.