Back to Search
Start Over
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway
- Source :
- Pharmaceutical Biology, Vol 60, Iss 1, Pp 1022-1031 (2022)
- Publication Year :
- 2022
- Publisher :
- Taylor & Francis Group, 2022.
-
Abstract
- Context Puerarin (Pue) and tanshinone IIA (Tan IIA) are often used in combination in the treatment of cerebrovascular diseases.Objective To investigate the neuroprotective effect and synergic mechanism of Pue-Tan IIA on the treatment of ischaemic stroke (IS).Materials and methods IS was induced in rats by middle cerebral artery occlusion (MCAO). Rats were intraperitoneally injected with Pue (36 mg/kg), Tan IIA (7.2 mg/kg), or Pue-Tan IIA (36 and 7.2 mg/kg) for five times [30 min before ischaemia, immediately after reperfusion (0 h), 24, 48, and 72 h after reperfusion]. After administration, neurological function assessment and histological changes in the brain were performed. S-100β and NSE levels were measured to determine the severity of brain injury. Oxidative stress parameters and inflammatory mediators were measured. The proteins involved in Nrf2/ARE signalling pathway were determined by qRT-PCR and western blot.Results After administration, the neurological function scores, infarct volume, S-100β, and NSE levels were significantly reduced in MCAO rats, especially with Pue-Tan IIA treatment (p
Details
- Language :
- English
- ISSN :
- 13880209 and 17445116
- Volume :
- 60
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Pharmaceutical Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.564dc87265c4fe5a2ccb9680406fb82
- Document Type :
- article
- Full Text :
- https://doi.org/10.1080/13880209.2022.2070221