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Buccal Administration of a Zika Virus Vaccine Utilizing 3D-Printed Oral Dissolving Films in a Mouse Model

Authors :
Sarthak Shah
Parth Patel
Amarae Ferguson
Priyal Bagwe
Akanksha Kale
Emmanuel Adediran
Revanth Singh
Tanisha Arte
Dedeepya Pasupuleti
Mohammad N. Uddin
Martin D’Souza
Source :
Vaccines, Vol 12, Iss 7, p 720 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Over the years, research regarding the Zika virus has been steadily increasing. Early immunization for ZIKV is a priority for preventing complications such as microencephaly and Guillain–Barré syndrome (GBS). Unlike traditional vaccination approaches, oral dissolving films (ODFs) or mucoadhesive film technology is an emerging, exciting concept that can be used in the field of pharmaceuticals for vaccine design and formulation development. This attractive and novel method can help patients who suffer from dysphagia as a complication of a disease or syndrome. In this study, we investigated a microparticulate Zika vaccine administered via the buccal route with the help of thin films or oral dissolving films (ODFs) with a prime dose and two booster doses two weeks apart. In vitro, the ODFs displayed excellent physiochemical properties, indicating that the films were good carriers for vaccine microparticles and biocompatible with the buccal mucosa. In vivo results revealed robust humoral (IgG, subtypes IgG1 and IgG2a) and T-cell responses (CD4+/CD8+) for ZIKV-specific immunity. Both the Zika MP vaccine and the adjuvanted Zika MP vaccine affected memory (CD45R/CD27) and intracellular cytokine (TNF-α and IL-6) expression. In this study, ZIKV vaccination via the buccal route with the aid of ODFs demonstrated great promise for the development of pain-free vaccines for infectious diseases.

Details

Language :
English
ISSN :
2076393X
Volume :
12
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Vaccines
Publication Type :
Academic Journal
Accession number :
edsdoj.568dfd90bd9d4f34829a52c9dcb4c041
Document Type :
article
Full Text :
https://doi.org/10.3390/vaccines12070720