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A genetically encoded fluorescent biosensor for extracellular l-lactate

Authors :
Yusuke Nasu
Ciaran Murphy-Royal
Yurong Wen
Jordan N. Haidey
Rosana S. Molina
Abhi Aggarwal
Shuce Zhang
Yuki Kamijo
Marie-Eve Paquet
Kaspar Podgorski
Mikhail Drobizhev
Jaideep S. Bains
M. Joanne Lemieux
Grant R. Gordon
Robert E. Campbell
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

l-lactate is an important intercellular energy currency. Here the authors report a genetically encoded biosensor eLACCO1.1 to monitor extracellular l-lactate; they use eLACCO1.1 to image extracellular l-lactate in cultured mammalian cells and brain tissue.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.56b5eb5b8e5543099fe7a7880622d7ac
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-27332-2