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Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype

Authors :
Hiroki Matsumoto
Masashi Miyagawa
Sayaka Takahashi
Ryouichi Shima
Takayuki Oosumi
Source :
Veterinary Sciences, Vol 7, Iss 3, p 106 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a major cause of post-weaning diarrhea (PWD) in pigs and causes significant damage to the swine industry worldwide. In recent years, there has been increased regulation against the use of antibacterial agents in swine due to their health risks. Utilizing experimental models that consistently recapitulate PWD is important for the development of non-antibacterial agents against PWD in pigs. In this study, we established a highly reproducible PWD infection model by examining differences in adhesion of ETEC to the intestinal tissue as well as the association between MUC4 polymorphisms and sensitivity to PWD. Post-weaning diarrhea differences between pig breeds were also examined. The adhesion to enterocytes varied from 104.0 to 106.4 CFU/mL even among the F4 ETEC strains. Experimental infection revealed that PWD can be induced in all MUC4 genotypes after infection with 1010 CFU/pig of highly adherent ETEC, although there were variable sensitivities between the genotypes. Lowly adherent ETEC did not cause PWD as efficiently as did highly adherent ETEC. The incidence of PWD was confirmed for all pigs with the ETEC-susceptible MUC4 genotypes in all of the breeds. These results indicate that high-precision and reproducible experimental infection is possible regardless of pig breeds by controlling factors on the pig-end (MUC4 genotype) and the bacterial-end (adhesion ability).

Details

Language :
English
ISSN :
23067381
Volume :
7
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Veterinary Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.57196996cf14484fb86c4838138cbfc3
Document Type :
article
Full Text :
https://doi.org/10.3390/vetsci7030106