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Development of Methodic Approach to Construction of Recombinant Strain, Producer of the Cholera Toxin B Subunit

Authors :
D. S. Yanov
S. N. Klinova
A. V. Mironin
I. V. Zhivov
I. P. Pogorelsky
V. I. Drobkov
Yu. N. Feodorov
Ya. A. Kibirev
Source :
Проблемы особо опасных инфекций, Vol 0, Iss 4(106), Pp 62-65 (2010)
Publication Year :
2010
Publisher :
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”, 2010.

Abstract

Presented are the results of experiments on the construction of Escherichia coli Top10 (pTrcSuB) strain, perspective for the development of recombinant cholera vaccine for peroral use. The obtained strain possesses inducible expression of cholera toxin B subunit. Peculiarities of cloned gene expression in strain-producer and the conditions of its maintaining were studied. The proposed approach will allow to obtain effective and safe cholera vaccines.

Details

Language :
Russian
ISSN :
03701069 and 2658719X
Issue :
4(106)
Database :
Directory of Open Access Journals
Journal :
Проблемы особо опасных инфекций
Publication Type :
Academic Journal
Accession number :
edsdoj.5748d708c94a47299f0b965cf9a2c974
Document Type :
article
Full Text :
https://doi.org/10.21055/0370-1069-2010-4(106)-62-65