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Influence of high density lipoprotein cholesterol levels on circulating monocytic angiogenic cells functions in individuals with type 2 diabetes mellitus

Authors :
Daniela Lucchesi
Simona Georgiana Popa
Veronica Sancho
Laura Giusti
Monia Garofolo
Giuseppe Daniele
Laura Pucci
Roberto Miccoli
Giuseppe Penno
Stefano Del Prato
Source :
Cardiovascular Diabetology, Vol 17, Iss 1, Pp 1-13 (2018)
Publication Year :
2018
Publisher :
BMC, 2018.

Abstract

Abstract Background High-density lipoproteins (HDLs) can exert anti-atherogenic effects. On top of removing excess cholesterol through reverse cholesterol transport, HDLs play beneficial actions on endothelial function and integrity. In particular, HDLs are strong determinant of endothelial progenitor cells (EPCs) number and function. To gain further insights into such an effect we characterized in vitro functionality of circulating “early” EPCs obtained from 60 type 2 diabetes individuals with low HDL-cholesterol (HDL-C) and 59 with high HDL-C levels. Methods After an overnight fast, venous blood was drawn in EDTA tubes and processed within 2-h from sampling. Peripheral blood mononuclear cells were isolated and plated on fibronectin coated culture dishes; after 3 days culture, adherent cells positive for Dil-ac-LDL/Lectin dual fluorescent staining were identified as monocytic angiogenic cells (MACs). After 5–7 days culture in EBM-2 medium, adherent cells were evaluated for viability/proliferation (MTT assay), senescence (beta-galactosidase activity detection), migration (modified Boyden chamber using VEGF as chemoattractant), adhesion capacity (on fibronectin-coated culture dishes) and ROS production (ROS-sensitive fluorescent probe CM-H2DCFDA). Results MACs obtained from diabetic individuals with high HDL-C had 23% higher viability compared to low HDL-C (111.6 ± 32.7% vs. 90.5 ± 28.6% optical density; p = 0.002). H2O2 exposure impaired MACs viability to a similar extent in both groups (109.2 ± 31.7% vs. 74.5 ± 40.8% in high HDL-C, p

Details

Language :
English
ISSN :
14752840
Volume :
17
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Cardiovascular Diabetology
Publication Type :
Academic Journal
Accession number :
edsdoj.578468615028404da305196bc5f7bd46
Document Type :
article
Full Text :
https://doi.org/10.1186/s12933-018-0720-1