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Genome-wide identification of MATE and ALMT genes and their expression profiling in mungbean (Vigna radiata L.) under aluminium stress

Authors :
Dharmendra Singh
Ankita Tripathi
Raktim Mitra
Jyotika Bhati
Varsha Rani
Jyoti Taunk
Deepti Singh
Rajendra Kumar Yadav
Manzer H. Siddiqui
Madan Pal
Source :
Ecotoxicology and Environmental Safety, Vol 280, Iss , Pp 116558- (2024)
Publication Year :
2024
Publisher :
Elsevier, 2024.

Abstract

The Multidrug and toxic compound extrusion (MATE) and aluminium activated malate transporter (ALMT) gene families are involved in response to aluminium (Al) stress. In this study, we identified 48 MATE and 14 ALMT gene families in Vigna radiata genome and classified into 5 (MATE) and 3 (ALMT) clades by phylogenetic analysis. All the VrMATE and VrALMT genes were distributed across mungbean chromosomes. Tandem duplication was the main driving force for evolution and expansion of MATE gene family. Collinearity of mungbean with soybean indicated that MATE gene family is closely linked to Glycine max. Eight MATE transporters in clade 2 were found to be associated with previously characterized Al tolerance related MATEs in various plant species. Citrate exuding motif (CEM) was present in seven VrMATEs of clade 2. Promoter analysis revealed abundant plant hormone and stress responsive cis-elements. Results from quantitative real time-polymerase chain reaction (qRT-PCR) revealed that VrMATE19, VrMATE30 and VrALMT13 genes were markedly up-regulated at different time points under Al stress. Overall, this study offers a new direction for further molecular characterization of the MATE and ALMT genes in mungbean for Al tolerance.

Details

Language :
English
ISSN :
01476513
Volume :
280
Issue :
116558-
Database :
Directory of Open Access Journals
Journal :
Ecotoxicology and Environmental Safety
Publication Type :
Academic Journal
Accession number :
edsdoj.58e9d8f24c204b43ba23eeae8d1c0ae1
Document Type :
article
Full Text :
https://doi.org/10.1016/j.ecoenv.2024.116558