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Identification of hub fatty acid metabolism-related genes and immune infiltration in IgA nephropathy

Authors :
Xiaoqian Qian
Shuyang Bian
Qin Guo
Dongdong Zhu
Fan Bian
Yinhui Song
Gengru Jiang
Source :
Renal Failure, Vol 46, Iss 2 (2024)
Publication Year :
2024
Publisher :
Taylor & Francis Group, 2024.

Abstract

Aims To investigate the potential mechanisms of fatty acid metabolism (FAM)-related genes in IgA nephropathy (IgAN) and to explore its immune cell infiltration characteristic.Methods Datasets for IgAN and FAM-related genes were obtained from GEO and MSigDB database, respectively. We employed differential expression analysis and WGCNA to identify common genes. GO and KEGG analyses were performed to compare the differences between IgAN and control groups. Furthermore, LASSO logistic regression was applied to develop a predictive model based on FAM-related genes. The efficacy of this prognostic model was evaluated using ROC analysis. The infiltration of immune cells and immune-related functions were assessed with CIBERSORT tool. Finally, the identified key genes were validated in blood samples from IgAN and control patients, as well as in human mesangial cells (HMCs) following Gd-IgA stimulation using Real-time PCR.Results A total of 12 hub genes linked to FAM were identified in patients with IgAN. A predictive model consisting of four genes was conducted through COX and LASSO regression analysis, revealing AUC values that indicate a relatively strong diagnostic capability. Immune infiltration analysis indicated that various immune cells have significant associations with IgAN. Additionally, Real-time PCR assays confirmed that the expression levels of hub genes were markedly reduced in IgAN patients and in Gd-IgA treated HMCs compared to controls.Conclusion This study employed bioinformatics methods to unveiled the immune cell infiltration associated with IgAN and to explore the potential genetic connection between FAM and IgAN. This could aid in predicting the risk of IgAN and enhance both diagnosis and prognosis of this condition.

Details

Language :
English
ISSN :
0886022X and 15256049
Volume :
46
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Renal Failure
Publication Type :
Academic Journal
Accession number :
edsdoj.595996a7e9264a71bd7767b4872b1fed
Document Type :
article
Full Text :
https://doi.org/10.1080/0886022X.2024.2427158