Back to Search Start Over

The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission in transgenic chickens

Authors :
Sung June Byun
Hoonsung Choi
Shanmugam Sureshkumar
Seong-Su Yuk
Jung-Hoon Kwon
Jin-Yong Noh
Sun Keun Jung
Jeom Sun Kim
Keon Bong Oh
Hyeon Yang
Gunsup Lee
Hwi-Cheul Lee
Jae-Seok Woo
Chang-Seon Song
Source :
BMC Veterinary Research, Vol 16, Iss 1, Pp 1-6 (2020)
Publication Year :
2020
Publisher :
BMC, 2020.

Abstract

Abstract Background The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission. Result The transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p

Details

Language :
English
ISSN :
17466148
Volume :
16
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Veterinary Research
Publication Type :
Academic Journal
Accession number :
edsdoj.5a394dc3dc304fcc980047ddf92e4779
Document Type :
article
Full Text :
https://doi.org/10.1186/s12917-020-02462-9