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TPC1-Type Channels in Physcomitrium patens: Interaction between EF-Hands and Ca2+
- Source :
- Plants, Vol 11, Iss 24, p 3527 (2022)
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- Two-pore channels (TPCs) are members of the superfamily of ligand-gated and voltage-sensitive ion channels in the membranes of intracellular organelles of eukaryotic cells. The evolution of ordinary plant TPC1 essentially followed a very conservative pattern, with no changes in the characteristic structural footprints of these channels, such as the cytosolic and luminal regions involved in Ca2+ sensing. In contrast, the genomes of mosses and liverworts encode also TPC1-like channels with larger variations at these sites (TPC1b channels). In the genome of the model plant Physcomitrium patens we identified nine non-redundant sequences belonging to the TPC1 channel family, two ordinary TPC1-type, and seven TPC1b-type channels. The latter show variations in critical amino acids in their EF-hands essential for Ca2+ sensing. To investigate the impact of these differences between TPC1 and TPC1b channels, we generated structural models of the EF-hands of PpTPC1 and PpTPC1b channels. These models were used in molecular dynamics simulations to determine the frequency with which calcium ions were present in a coordination site and also to estimate the average distance of the ions from the center of this site. Our analyses indicate that the EF-hand domains of PpTPC1b-type channels have a lower capacity to coordinate calcium ions compared with those of common TPC1-like channels.
- Subjects :
- TPC1
vacuole
EF-domain
Physcomitrella
coordination of Ca2+
Botany
QK1-989
Subjects
Details
- Language :
- English
- ISSN :
- 22237747
- Volume :
- 11
- Issue :
- 24
- Database :
- Directory of Open Access Journals
- Journal :
- Plants
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.5a7494c3733c4a87bd2025c86a54ddaf
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/plants11243527