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Molecular Characterisation and Antibody Response to Bovine Respiratory Syncytial Virus in Vaccinated and Infected Cattle in Turkey
- Source :
- Pathogens, Vol 13, Iss 4, p 304 (2024)
- Publication Year :
- 2024
- Publisher :
- MDPI AG, 2024.
-
Abstract
- Bovine respiratory syncytial virus (BRSV) is one of the most important respiratory pathogens of cattle. In this study, frequency of infection, analysis of variants, and the immune status of vaccinated and non-vaccinated cattle were studied. Blood (n = 162) and nasal/oropharyngeal (n = 277) swabs were collected from 62 cattle herds in Turkey. Lung samples (n = 37) were also taken from dead animals and abattoirs. Antibodies to BRSV were detected in 76 (46%) out of 162 sera. The antibody levels in the vaccinated and non-vaccinated groups were statistically significant. Among 277 nasal/oropharyngeal swabs and 37 lungs, ten nasal/oropharyngeal and four lung samples were positive for BRSV-RNA. BRSV-G gene sequences of 5 out of 14 RT-PCR positive samples showed that all viruses clustered as Group-III in phylogenetic analysis with 88–100% homology. Similarity with previous Turkish BRSVs was 89–98%, and that with BRSVs detected in the USA and Czechia was 89.47–93.12%. BRSV continues to circulate in Turkish cattle, and vaccination seems beneficial in preventing BRSV. The diversity of the BRSVs found in this study needs be considered in vaccination strategies.
- Subjects :
- bovine respiratory syncytial virus
antibody
ELISA
PCR
phylogenetic
cattle
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 13
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.5b039365ee4a48dd9e33f2eb454ee0a4
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/pathogens13040304