Expression and clinical significance of ASH2L in human gastric carcinoma cell lines infected with Helicobacter pylori

Objective To explore the expression and clinical significance of Drosophila protein Ash2 analogue(ASH2L)in gastric carcinoma cell lines infected with Helicobacter pylori(Hp). Methods The expression of ASH2L in various tumors, the relationship between ASH2L and pathological characteristics of stomach adenocarcinoma and the prognosis of the patients were analyzed by TIMER2.0, UALCAN and other software. Human gastric mucosal epithelial cell line GES, gastric carcinoma cell lines SGC-7901 and AGS were infected with Hp and the expression of ASH2L before and after Hp infection was detected by RT-qPCR and Western blot. Results The analysis showed that ASH2L was differentially expressed in 15 kinds of tumors and was highly expressed in stomach adenocarcinoma. The overall survival (OS) and post-progression survival (PPS) of patients with high ASH2L expression were lower than those with low ASH2L expression(P＜0.05). There were some differences in the expression of ASH2L in patients with stomach adenocarcinoma in different age groups, different races and different pathological grades, but not related to gender. The expression of ASH2L was significantly up-regulated in three human gastric epithelial cell lines infected with Hp(P＜0.05). Conclusions ASH2L may play an important role in tumorigenesis of gastric carcinoma caused by Hp infection, and its expression level is closely related to various pathological features and prognosis of the patients, and it is expected to be a molecular marker for prognosis.