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Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System

Authors :
Keke Chang
Ruipeng Chen
Shun Wang
Jianwei Li
Xinran Hu
Hao Liang
Baiqiong Cao
Xiaohui Sun
Liuzheng Ma
Juanhua Zhu
Min Jiang
Jiandong Hu
Source :
Sensors, Vol 15, Iss 8, Pp 20511-20523 (2015)
Publication Year :
2015
Publisher :
MDPI AG, 2015.

Abstract

The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 105 ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10−5 refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system.

Details

Language :
English
ISSN :
14248220
Volume :
15
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Sensors
Publication Type :
Academic Journal
Accession number :
edsdoj.5da5ed6e208e40bc9437f90b90b1e80e
Document Type :
article
Full Text :
https://doi.org/10.3390/s150820511