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An optimized protocol for PD-L1 pathological assessment with patient sample deglycosylation to improve correlation with therapeutic response
- Source :
- STAR Protocols, Vol 3, Iss 1, Pp 101198- (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier, 2022.
-
Abstract
- Summary: Immunotherapy via PD-1/PD-L1 blockade is a promising strategy to eradicate cancer cells. However, the PD-L1 pathological level is inconsistent with the therapeutic response and is not a reliable biomarker to stratify patients for anti-PD-1/PD-L1 therapy. Here, we describe patient sample deglycosylation in an immunohistochemistry (IHC) assay to resolve this challenge. This protocol facilitates antigen retrieval by removing N-glycans from surface antigens on formalin-fixed paraffin-embedded (FFPE) tissue slides and can be applied in medical pathology for multiple cancer types.For complete details on the use and execution of this profile, please refer to Lee et al. (2019).
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 3
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.5ee966d3eadc4c6cabc5f6119e5cb1fd
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2022.101198