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Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

Authors :
Ingrid Chamma
Mathieu Letellier
Corey Butler
Béatrice Tessier
Kok-Hong Lim
Isabel Gauthereau
Daniel Choquet
Jean-Baptiste Sibarita
Sheldon Park
Matthieu Sainlos
Olivier Thoumine
Source :
Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016)
Publication Year :
2016
Publisher :
Nature Portfolio, 2016.

Abstract

The advent of fluorescence-based super-resolution microscopy has created a need for labeling strategies relying on small probes that minimally perturb protein function. Here the authors describe a labeling method that reduces protein tag and label sizes, allowing for accurate protein targeting and measurements of protein dynamics in tight cellular spaces.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
7
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.5f33096ea5754f40ac84a9bb09f90a56
Document Type :
article
Full Text :
https://doi.org/10.1038/ncomms10773