Dna damage in lymphocytes in chronic viral hepatitis B, C

Objective: determine grade of DNA damage in lymphocytes and the levels of 8-hydroxy-2-deoxyguanosine (8- OHdG) and 8-nitroguanine (8-NO2G), total antioxidant activity (TAA) in the blood serum of patients with chronic viral hepatitis C (HCV), B (HBV).Materials and methods. The study included 100 people with HCV, 50 with HBV and 43 volunteers. DNA damage was evaluated by comet assay. Level 8-NO2G and 8-OHdG were determined in serum samples using ELISA kits. TAA determined according to the standard ABTS method. All statisticalcalculations were performed and the graphs were plotted using STATISTICA 10.0 software.Results. There was a tendency to increase the level of DNA damage with progression of the liver fibrosis stage in HCV and HBV. Significant differences from the norm of 8-NO2G in the F1, F2, F3 groups with HCV and in the groups F2, F3, F4 (ρ