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Generation of human induced pluripotent stem cell (iPSC) line from an unaffected female carrier of mutation in SACSIN gene

Authors :
Candela Machuca
Angel Vilches
Eleonora Clemente
Samuel Ignacio Pascual-Pascual
Arantxa Bolinches-Amorós
Ana Artero Castro
Carmen Espinos
Marian Leon
Pavla Jendelova
Slaven Erceg
Source :
Stem Cell Research, Vol 33, Iss , Pp 166-170 (2018)
Publication Year :
2018
Publisher :
Elsevier, 2018.

Abstract

The human iPSC cell line, CARS-FiPS4F1 (ESi064-A), derived from dermal fibroblast from the apparently healthy carrier of the mutation of the gene SACSIN, was generated by non-integrative reprogramming technology using OCT3/4, SOX2, CMYC and KLF4 reprogramming factors. The pluripotency was assessed by immunocytochemistry and RT-PCR. This iPSC line can be used as control for Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) disease.

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
18735061
Volume :
33
Issue :
166-170
Database :
Directory of Open Access Journals
Journal :
Stem Cell Research
Publication Type :
Academic Journal
Accession number :
edsdoj.6099d90be0f440dab9874731f3220b0f
Document Type :
article
Full Text :
https://doi.org/10.1016/j.scr.2018.10.016