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Paeonol inhibits profibrotic signaling and HOTAIR expression in fibrotic buccal mucosal fibroblasts

Paeonol inhibits profibrotic signaling and HOTAIR expression in fibrotic buccal mucosal fibroblasts

Authors :
Dennis Chun-Yu Ho
Szu-Han Chen
Chih-Yuan Fang
Chang-Wei Hsieh
Pei-Ling Hsieh
Yi-Wen Liao
Cheng-Chia Yu
Lo-Lin Tsai
Source :
Journal of the Formosan Medical Association, Vol 121, Iss 5, Pp 930-935 (2022)
Publication Year :
2022
Publisher :
Elsevier, 2022.

Abstract

Background/purpose: Betel nut chewing is the major risk factor of oral submucous fibrosis (OSF). Various studies have sought to discover alternative strategies to alleviate oral fibrogenesis. In the present study, we aimed to evaluate the anti-fibrosis effect of paeonol, a phenolic component derived from Paeonia Suffruticosa. Methods: The cytotoxicity of paeonol was tested using normal and fibrotic buccal mucosal fibroblasts (fBMFs) derived from OSF tissues. Collagen gel contraction, Transwell migration, invasion, and wound healing capacities were examined. Besides, the activation of TGF-β/Smad2 signaling and expression levels of type I collagen, α-SMA, and long non-coding RNA HOTAIR were measured as well. Results: Paeonol exerted a higher cytotoxic effect on fBMFs compared to normal BMFs. The arecoline-induced myofibroblast activities, including collagen gel contractility, cell motility, and wound healing ability were all suppressed by paeonol treatment. In addition, the activation of the TGF-β/Smad2 pathway was inhibited along with a lower expression of α-SMA and type I collagen in paeonol-treated cells. Also, the administration of paeonol decreased the mRNA expression of HOTAIR in fBMFs. Conclusion: Our results indicate that paeonol may be a promising compound to attenuate the progression of oral fibrogenesis in OSF patients.

Details

Language :
English
ISSN :
09296646
Volume :
121
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Journal of the Formosan Medical Association
Publication Type :
Academic Journal
Accession number :
edsdoj.61ec04973bfb4ab0b3f9727b16e442b6
Document Type :
article
Full Text :
https://doi.org/10.1016/j.jfma.2021.09.027