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Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition by N-Acetyl-pentapeptides
- Source :
- The Scientific World Journal, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation of L-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC50 0.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.
- Subjects :
- Technology
Medicine
Science
Subjects
Details
- Language :
- English
- ISSN :
- 23566140 and 1537744X
- Volume :
- 2014
- Database :
- Directory of Open Access Journals
- Journal :
- The Scientific World Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.621911a9788b4587848a9c4ad37e3791
- Document Type :
- article
- Full Text :
- https://doi.org/10.1155/2014/409783