Chrysanthemum morifolium extract mediated Ag NPs improved the cytotoxicity effect in A549 lung cancer cells

In this study, the anti-cancer compound was extracted from Chrysanthemum morifolium (C. morifolium) by soxhlet apparatus method. The available anti-cancer compounds of Chrysanthemum morifolium extract were clearly detected by LC-MS analysis based on the retention time, percentages of height and occupied area. The anti-cancer compounds were separately screened from mixed compounds by NIST Wiley library interpretation. Further, the silver nanoparticle (Ag NPs) was synthesized from C. morifolium extract, and their surface plasmon resonance effect of the extract was scanned by UV–vis spectroscopy at 410 nm. Morphological identification of size and shape of the Ag NPs was confirmed by scanning electron microscope and transmission electron microscope images. Further, the anti-cancer effect of synthesized Ag NPs was exhibited more cytotoxicity effect at the concentration of 200 µg/mL. Furthermore, the morphological damages of A549 cells were clearly confirmed by fluorescence microscope using AO/EB fluorescence stain. Finally, the results were suggested that the biosynthesized Ag NPs has potential anti-cancer agent against A549 lung cancer cells.