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Inhibition of the norA gene expression and the NorA efflux pump by the tannic acid

Authors :
Saulo Relison Tintino
Polrat Wilairatana
Veruska Cintia Alexandrino de Souza
Julia Mariana Assis da Silva
Pedro Silvino Pereira
Cícera Datiane de Morais Oliveira-Tintino
Yedda Maria Lobo Soares de Matos
João Tavares Calixto Júnior
Valdir de Queiroz Balbino
José P. Siqueira-Junior
Irwin Rose Alencar Menezes
Abolghasem Siyadatpanah
Henrique Douglas Melo Coutinho
Tereza Cristina Leal Balbino
Source :
Scientific Reports, Vol 13, Iss 1, Pp 1-9 (2023)
Publication Year :
2023
Publisher :
Nature Portfolio, 2023.

Abstract

Abstract The NorA efflux pump of Staphylococcus aureus is known to play a major role in the development of resistance against quinolone drugs by reducing their concentration inside target pathogens. The objective of this study was to evaluate the ability of tannic acid to inhibit the gene expression of the NorA efflux pump in Staphylococcus aureus and to evaluate the in silico effect on the pump. Efflux pump inhibition was evaluated by fluorimetry. The checkerboard method evaluates the effect of the test substance in combination with an antimicrobial at different concentrations. To gene expression evaluation NorA the assay was performed using: a sub-inhibitory concentration preparation (MIC/4) of the antibiotic; a sub-inhibitory concentration preparation (MIC/4) of the antibiotic associated with tannic acid at a sub-inhibitory concentration (MIC/4). In this study, docking simulations were performed by the SWISSDOCK webserver. The ability of tannic acid to inhibit the NorA efflux pump can be related to both the ability to inhibit the gene expression of this protein, acting on signaling pathways involving the ArlRS membrane sensor. As well as acting directly through direct interaction with the NorA protein, as seen in the approach and in silico and in vitro per checkerboard method and fluorimetry of bromide accumulated in the cell.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
20452322
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.625ce5366c264773b240a845fd3f10f5
Document Type :
article
Full Text :
https://doi.org/10.1038/s41598-023-43038-5