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A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses

Authors :
Graziela Alves da Cunha Valini
Pedro Righetti Arnaut
Larissa Gonçalves Barbosa
Paulo Henrique Amadeu de Azevedo
Antonio Diego Brandão Melo
Danilo Alves Marçal
Paulo Henrique Reis Furtado Campos
Luciano Hauschild
Source :
Microorganisms, Vol 11, Iss 2, p 446 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Salmonella Typhimurium is the most frequent serovar in pigs and causes infections in humans. However, the dosage used for experimentation is not well defined. The present study aimed to evaluate a dosage for oral inoculation with Salmonella Typhimurium to assess immunological and growth performance alterations in pigs. Gilts were randomly allocated into one of three experimental treatments: no Salmonella Typhimurium inoculation (Basal), or oral inoculation of 1 × 108 or 1.5 × 108 colony-forming units of Salmonella Typhimurium. Growth rate, rectal temperature, and fecal Salmonella shedding were recorded. Blood samples were taken. Inoculated pigs shed the bacteria for up to 7 days, but no differences were observed between the groups. No differences were observed in rectal temperature, body weight, or average daily feed intake. However, reductions in average daily gain (−17 and −22%) and feed efficiency (−14 and −20%) were observed in pigs inoculated with 1 × 108 and 1.5 × 108 colony-forming units, respectively. The hemoglobin and hematocrit concentrations increased in challenged pigs compared to Basal pigs. The oral dosage of 1.5 × 108 colony-forming units of Salmonella Typhimurium is suitable for activating the immune system of pigs and assessing the impact of Salmonella on pig performance.

Details

Language :
English
ISSN :
20762607
Volume :
11
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.628da68607a45dc891182d13a4b2b7e
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms11020446