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Identification and Characterization of GYS and GSK3β Provides Insights into the Regulation of Glycogen Synthesis in Jinjiang Oyster Crassostrea ariakensis

Authors :
Yan Wang
Zhihong Liu
Xi Chen
Liqing Zhou
Xiujun Sun
Tao Yu
Xiaomei Wang
Yanxin Zheng
Biao Wu
Source :
Fishes, Vol 8, Iss 2, p 65 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Glycogen, a stored form of glucose, is an important form of energy for aquatic shellfish, contributing to the flavor and quality of the oyster. The glycogen synthase (GYS) and glycogen synthase kinase 3β (GSK3β) are two major enzymes in the glycogenesis. However, the information of the two genes in the Jinjiang oyster Crassostrea ariakensis remains limited. In this study, we identified the genes of GYS and GSK3β and further explored their function in the glycogen synthesis of C. ariakensis. The GYS and GSK3β were distributed in all tested tissues, and high expression of GYS and glycogen content were detected in the gonad, labial palp, hepatopancreas, and mantle, while the high expression of GSK3β was observed in the gill and adductor muscle. The expression of GYS was positively correlated with the glycogen content, while GSK3β was negatively correlated. Additionally, knockdown of GSK3β using RNAi decreased the GYS expression, revealing the negative regulatory effect of GSK3β on GYS. These findings enrich the research data of GSK3β and GYS involved in glycogen synthesis, providing valuable information for further research on the function of GSK3β and GYS in the glycogen synthesis process of oyster.

Details

Language :
English
ISSN :
24103888
Volume :
8
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Fishes
Publication Type :
Academic Journal
Accession number :
edsdoj.629f7bbb514438e902f8dbbe53f942f
Document Type :
article
Full Text :
https://doi.org/10.3390/fishes8020065