Cryptosporidium Soil Extraction by Filtration/IMS/FA Compatible with USEPA Method 1623.1

a parasitic pathogen, when present in drinking water supplies poses a significant risk to human health. The soil environment provides a critical linkage between parent material (bovine manure) and the surface and groundwaters used by susceptible human populations for drinking water. The lack of a USEPA published analysis for the determination of in soil is a barrier to standardized investigation of transport in soils. Methodologies have been published in the absence of a USEPA analysis, yet they lack sufficient detail and quality controls to be generally applicable. This study developed and evaluated a soil extraction method capable of producing an extracted fluid containing that could be purified and enumerated using USEPA Method 1623.1.