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Exploring the impact of 1-deoxynojirimycin on alpha-galactosidase activity and chickpea seed germination through in vitro experiments and molecular docking analysis
- Source :
- Journal of Seed Science, Vol 45 (2023)
- Publication Year :
- 2023
- Publisher :
- Associação Brasileira de Tecnologia de Sementes, 2023.
-
Abstract
- ABSTRACT: Seed germination is a tightly regulated physiological process. Hydrolytic enzymes provide energy that brings physiological, biochemical, and physical changes to the seed during germination. Alpha-galactosidases break down alpha 1-6 linked galactosyl groups in glycoproteins, glycolipids, and oligosaccharides into simple compounds to provide energy during seed germination. Chickpea seed germination characteristics showed that inhibition of alpha-galactosidase using 1-deoxynojirimycin (DNJ), a derivative of deoxygalactonojirimycin, delays germination. To gain insights into the mechanism of inhibition, we modeled the enzyme’s structure and performed in-silico docking of the inhibitor and natural substrates (raffinose and stachyose) to the enzyme. We also validated our model using recombinant chickpea alpha-galactosidase. Our docking studies showed that both the inhibitor and the substrates bind to the same active site pocket in the enzyme but to different amino acid residues, stachyose being a preferred substrate, and DNJ competitively inhibits alpha-galactosidase with a Ki and IC50 of 44.5 µM and 89.13 µM respectively. Delayed germination is a desirable agronomic practice that can be explored for better crop establishment and to prevent pre-harvest sprouting in crops.
Details
- Language :
- English
- ISSN :
- 23171545
- Volume :
- 45
- Database :
- Directory of Open Access Journals
- Journal :
- Journal of Seed Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6300b05fda0c4c8baa6fc6c681a7bace
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/2317-1545v45273706