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Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917
- Source :
- Synthetic and Systems Biotechnology, Vol 9, Iss 1, Pp 165-175 (2024)
- Publication Year :
- 2024
- Publisher :
- KeAi Communications Co., Ltd., 2024.
-
Abstract
- The probiotic bacterium Escherichia coli Nissle 1917 (EcN) holds significant promise for use in clinical and biological industries. However, the reliance on antibiotics to maintain plasmid-borne genes has overshadowed its benefits. In this study, we addressed this issue by engineering the endogenous cryptic plasmids pMUT1 and pMUT2. The non-essential elements were removed to create more stable derivatives pMUT1NR△ and pMUT2HBC△. Synthetic promoters by integrating binding motifs on sigma factors were further constructed and applied for expression of Bacteroides thetaiotaomicron heparinase III and the biosynthesis of ectoine. Compared to traditional antibiotic-dependent expression systems, our newly constructed antibiotic-free expression systems offer considerable advantages for clinical and synthetic biology applications.
Details
- Language :
- English
- ISSN :
- 2405805X
- Volume :
- 9
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Synthetic and Systems Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.6322c0c9fe4ed7a3186e793c9ddc16
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.synbio.2024.01.006