Synergistic effects of royal jelly and glycine on the healing of skin wounds in mice

Aims:This study aims to assess how the synergistic effects of royal jelly (RJ) and glycine, which are easily accessible and economically viable substances, can enhance the restoration of skin affected by wounds. This study provides valuable insights into pioneering strategies for addressing the critical concern of effectively treating skin wounds.Methods:The experimental design of this study involved the formulation of different concentrations of RJ and glycine. A total of 80 male NMARI mice were categorized into two groups of 10, each following specific oral and topical treatment protocols. Additionally, a 5-mm diameter wound was created on the back of the neck. These wounds were then treated orally and topically with varying doses of RJ and glycine, and their combination, over 9 days. Wound measurements were taken and recorded daily throughout the study period. On the 10th day, mice were anesthetized under ethical conditions, and skin tissue samples were collected for subsequent histological examinations and hydroxyproline measurements.Results:The synergistic effects of combined oral treatment with RJ (50 mg/kg) and glycine (3 and 12 mg/kg) and simultaneous topical treatment of RJ (2.5%) and glycine (0.2 and 1%) caused a significant reduction in skin wound diameter (p