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A biosensor-based framework to measure latent proteostasis capacity

Authors :
Rebecca J. Wood
Angelique R. Ormsby
Mona Radwan
Dezerae Cox
Abhishek Sharma
Tobias Vöpel
Simon Ebbinghaus
Mikael Oliveberg
Gavin E. Reid
Alex Dickson
Danny M. Hatters
Source :
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Publication Year :
2018
Publisher :
Nature Portfolio, 2018.

Abstract

A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.6391112b918e4b36a3854c8ea904fe80
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-017-02562-5