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Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Authors :
Ana Carolina Costa Santos
Serena Mares Malta
Raquel Cristina Cavalcanti Dantas
Nina Dias Coelho Rocha
Vasco Ariston de Carvalho Azevedo
Carlos Ueira-Vieira
Source :
BMC Microbiology, Vol 22, Iss 1, Pp 1-9 (2022)
Publication Year :
2022
Publisher :
BMC, 2022.

Abstract

Abstract Background The discovery of new molecules with antimicrobial properties has been a promising approach, mainly when related to substances produced by bacteria. The use of substances produced by bees has evidenced the antimicrobial action in different types of organisms. Thus, the use of bacteria isolated from larval food of stingless bees opens the way for the identification of the new molecules. The effect of supernatants produced by these bacteria was evaluated for their ability to inhibit the growth of bacteria of clinical interest. Furthermore, their effects were evaluated when used in synergy with antibiotics available in the pharmaceutical industry. Results A few supernatants showed an inhibitory effect against susceptible and multiresistant strains in the PIC assay and the modulation assay. Emphasizing the inhibitory effect on multidrug-resistant strains, 7 showed an effect on multidrug-resistant Escherichia coli (APEC), Klebsiella pneumoniae carbapenemase (KPC), multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Staphylococcus aureus (MRSA) in the PIC assay. Of the supernatants analyzed, some presented synergism for more than one species of multidrug-resistant bacteria. Nine had a synergistic effect with ampicillin on E. coli (APEC) or S. aureus (MRSA), 5 with penicillin G on E. coli (APEC) or KPC, and 3 with vancomycin on KPC. Conclusion In summary, the results indicate that supernatants produced from microorganisms can synthesize different classes of molecules with potent antibiotic activity against multiresistant bacteria. Thus, suggesting the use of these microorganisms for use clinical tests to isolate the molecules produced and their potential for use.

Details

Language :
English
ISSN :
14712180
Volume :
22
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Microbiology
Publication Type :
Academic Journal
Accession number :
edsdoj.644b51e4cd844fbbcd5cbefe37395b8
Document Type :
article
Full Text :
https://doi.org/10.1186/s12866-022-02548-4