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Combined inhibitory effect of interferon and antiserum on avian hepatitis E virus

Authors :
Hongjian Yan
Wanli Sun
Jinjin Wang
Qiming Gao
Yawen Zhang
Yixin Wang
Shuang Chang
Peng Zhao
Source :
Poultry Science, Vol 102, Iss 5, Pp 102591- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

ABSTRACT: Epidemiologic investigations in recent years have shown that the detection rate of avian hepatitis E virus (HEV) in chicken flocks is increasing in China. Nevertheless, effective prevention and control measures are still lacking. In this study, specific pathogen-free (SPF) chicken serum against HEV was prepared using recombinant HEV open reading frames (ORF2 and ORF3) proteins as immunogens. An SPF chicken infection model was established by intravenous inoculation of chick embryos. Swab samples were collected at 7, 14, 21, and 28 d of age and used to detect avian HEV load, along with other indicators, by fluorescence quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay. The therapeutic effects on blocking vertical HEV transmission were observed, by using the methods of antibody application alone, mixed, or combined application of each of the 2 antibodies with type I interferon. The results showed that type I interferon alone or in combination with antiserum reduced the positive rate of HEV from 100 to 62.5% and 25%, respectively. However, the avian HEV-positivity rate was reduced to 75, 50, and 37.5% after type I interferon was used alone or in combination with antisera against ORF2 and ORF3, respectively. The inhibitory effect of type I interferon alone or in combination with an antiserum, on HEV replication was more significant in cells than in vivo. In this study, the inhibitory effect of type I interferon alone or in combination with an antiserum on avian HEV replication was observed in vitro and in vivo, providing the necessary technical reserve for disease prevention and control.

Details

Language :
English
ISSN :
00325791
Volume :
102
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Poultry Science
Publication Type :
Academic Journal
Accession number :
edsdoj.64507d7c77104e63b4ad62983e46c8a4
Document Type :
article
Full Text :
https://doi.org/10.1016/j.psj.2023.102591