Back to Search Start Over

Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.

Authors :
Se Hwan Hwang
WeonSun Lee
Sang Hi Park
Hee Jin Lee
Sun Hwa Park
Dong Chang Lee
Mi Hyun Lim
Sang A Back
Byeong Gon Yun
Jung Ho Jeun
Jung Yeon Lim
Jun Myung Kang
Sung Won Kim
Source :
PLoS ONE, Vol 12, Iss 10, p e0186249 (2017)
Publication Year :
2017
Publisher :
Public Library of Science (PLoS), 2017.

Abstract

We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
19326203 and 47849908
Volume :
12
Issue :
10
Database :
Directory of Open Access Journals
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
edsdoj.64608f865b9a47849908ab65b0a55be1
Document Type :
article
Full Text :
https://doi.org/10.1371/journal.pone.0186249