Back to Search Start Over

In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts

Authors :
Joo Kim
Hak Soo Seo
Source :
Bio-Protocol, Vol 8, Iss 7 (2018)
Publication Year :
2018
Publisher :
Bio-protocol LLC, 2018.

Abstract

Nitrate reductase (NR) reduces the major plant nitrogen source, NO3-, into NO2-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present a detailed protocol for measuring relative enzyme activity of NR from Arabidopsis crude extracts. This protocol offers simple procedure and data analysis to compare NR activity of multiple samples.

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
23318325
Volume :
8
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Bio-Protocol
Publication Type :
Academic Journal
Accession number :
edsdoj.65a611a0803f4cc49dff2cf01fbfb72b
Document Type :
article
Full Text :
https://doi.org/10.21769/BioProtoc.2785