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In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts
- Source :
- Bio-Protocol, Vol 8, Iss 7 (2018)
- Publication Year :
- 2018
- Publisher :
- Bio-protocol LLC, 2018.
-
Abstract
- Nitrate reductase (NR) reduces the major plant nitrogen source, NO3-, into NO2-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present a detailed protocol for measuring relative enzyme activity of NR from Arabidopsis crude extracts. This protocol offers simple procedure and data analysis to compare NR activity of multiple samples.
- Subjects :
- Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 23318325
- Volume :
- 8
- Issue :
- 7
- Database :
- Directory of Open Access Journals
- Journal :
- Bio-Protocol
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.65a611a0803f4cc49dff2cf01fbfb72b
- Document Type :
- article
- Full Text :
- https://doi.org/10.21769/BioProtoc.2785