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A YAP/TAZ–ARHGAP29–RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions

Authors :
Manuel Rogg
Jasmin I. Maier
Martin Helmstädter
Alena Sammarco
Felix Kliewe
Oliver Kretz
Lisa Weißer
Clara Van Wymersch
Karla Findeisen
Anna L. Koessinger
Olga Tsoy
Jan Baumbach
Markus Grabbert
Martin Werner
Tobias B. Huber
Nicole Endlich
Oliver Schilling
Christoph Schell
Source :
Cells, Vol 12, Iss 13, p 1795 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

Glomerular disease due to podocyte malfunction is a major factor in the pathogenesis of chronic kidney disease. Identification of podocyte-specific signaling pathways is therefore a prerequisite to characterizing relevant disease pathways and developing novel treatment approaches. Here, we employed loss of function studies for EPB41L5 (Yurt) as a central podocyte gene to generate a cell type-specific disease model. Loss of Yurt in fly nephrocytes caused protein uptake and slit diaphragm defects. Transcriptomic and proteomic analysis of human EPB41L5 knockout podocytes demonstrated impaired mechanotransduction via the YAP/TAZ signaling pathway. Further analysis of specific inhibition of the YAP/TAZ-TEAD transcription factor complex by TEADi led to the identification of ARGHAP29 as an EPB41L5 and YAP/TAZ-dependently expressed podocyte RhoGAP. Knockdown of ARHGAP29 caused increased RhoA activation, defective lamellipodia formation, and increased maturation of integrin adhesion complexes, explaining similar phenotypes caused by loss of EPB41L5 and TEADi expression in podocytes. Detection of increased levels of ARHGAP29 in early disease stages of human glomerular disease implies a novel negative feedback loop for mechanotransductive RhoA—YAP/TAZ signaling in podocyte physiology and disease.

Details

Language :
English
ISSN :
20734409
Volume :
12
Issue :
13
Database :
Directory of Open Access Journals
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
edsdoj.65be523bab45463eaad86d94233ee798
Document Type :
article
Full Text :
https://doi.org/10.3390/cells12131795