Micropropagation to Eucalyptus grandis (Hill ex Maiden from nodals segments

With the purpose of developing a methodology for the propagation in vitro of Eucalyptus grandis (former Hill Maiden), they were carried out investigations that embraced from the pretratamiento of the donating plants until the evaluation of the plants in vitro in the field. The results showed that the previous application of fungicides and insecticides to the plants donating 21-30 days before the establishment it increased the efficiency of this phase, in which the best results in the disinfection were obtained with the application of hipoclorito of sodium to 1.0% during 10 minutes. With the addition of 6-bencilaminopurina and sour naftalenacético (0.1 mg.l-1) in the multiplication phase buds of more quality were obtained, that showed 75% rooting when they were individualized and transferred to a means of cultivation with 0.3 mg.l-1 of AIB. The evaluations carried out in the field to the in vitro plants demonstrated a dynamics of very superior growth to those taken place starting from seeds. Key words: multiplication, rooting, in vitro plant