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The Ursolic Acid-Rich Extract of Dracocephalum heterophyllum Benth. with Potent Antidiabetic and Cytotoxic Activities

Authors :
Sodik Numonov
Farukh Sharopov
Muhammad Nasimullah Qureshi
Latofat Gaforzoda
Isomiddin Gulmurodov
Qosimjon Khalilov
William N Setzer
Maidina Habasi
Haji Akber Aisa
Source :
Applied Sciences, Vol 10, Iss 18, p 6505 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Pentacyclic triterpenoids are one of the main functional components in Dracocephalum heterophyllum. In this study the optimal process, the fairly simple and accessible extraction and purification of triterpenoids of D. heterophyllum, was developed by a remaceration method. Remaceration is characterized by minimal loss of biologically active compounds on diffusion, which contributes to the largest depletion of raw materials. The triterpenoid yield was 2.4% under optimal conditions which was enhanced to 98.03% after purification. The triterpenoid profiles and their anticancer and antidiabetic activities were further analyzed. GC-MS analysis of triterpenoidal extract of D. heterophyllum resulted ursolic acid (71.9%) and oleanolic acid (18.1%) as the major components. Additionally, total purified triterpenoid contents of D. heterophyllum and its main components were shown to possess significant cytotoxic activity against three human breast cancer cell lines (SK-Br-3, T47D, and MCF-7). The purification of triterpenoids influenced their biological activity. The antidiabetic effect, as measured by inhibition of protein-tyrosine phosphatase (PTP-1B), of the purified fraction of triterpenoids of D. heterophyllum increased by five-fold against the enzyme. The results provide important guidance for the industrial application of D. heterophyllum confirming the prospect of developing plant extracts into effective drugs and health foods for human applications.

Details

Language :
English
ISSN :
20763417
Volume :
10
Issue :
18
Database :
Directory of Open Access Journals
Journal :
Applied Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.66ff484f6e7947a69101e127a120df9f
Document Type :
article
Full Text :
https://doi.org/10.3390/app10186505