Sorry, I don't understand your search. ×
Back to Search Start Over

Shiga-Toxin-Producing Strains of Escherichia coli O104:H4 and a Strain of O157:H7, Which Can Cause Human Hemolytic Uremic Syndrome, Differ in Biofilm Formation in the Presence of CO2 and in Their Ability to Grow in a Novel Cell Culture Medium

Authors :
Kei Amemiya
David A. Rozak
Jennifer L. Dankmeyer
William R. Dorman
Charles Marchand
David P. Fetterer
Patricia L. Worsham
Brett K. Purcell
Source :
Microorganisms, Vol 11, Iss 7, p 1744 (2023)
Publication Year :
2023
Publisher :
MDPI AG, 2023.

Abstract

One pathogen that commonly causes gastrointestinal illnesses from the consumption of contaminated food is Escherichia coli O157:H7. In 2011 in Germany, however, there was a prominent outbreak of bloody diarrhea with a high incidence of hemolytic uremic syndrome (HUS) caused by an atypical, more virulent E. coli O104:H4 strain. To facilitate the identification of this lesser-known, atypical E. coli O104:H4 strain, we wanted to identify phenotypic differences between it and a strain of O157:H7 in different media and culture conditions. We found that E. coli O104:H4 strains produced considerably more biofilm than the strain of O157:H7 at 37 °C (p = 0.0470–0.0182) Biofilm production was significantly enhanced by the presence of 5% CO2 (p = 0.0348–0.0320). In our study on the innate immune response to the E. coli strains, we used HEK293 cells that express Toll-like receptors (TLRs) 2 or 4. We found that E. coli O104:H4 strains had the ability to grow in a novel HEK293 cell culture medium, while the E. coli O157:H7 strain could not. Thus, we uncovered previously unknown phenotypic properties of E. coli O104:H4 to further differentiate this pathogen from E. coli O157:H7.

Details

Language :
English
ISSN :
20762607
Volume :
11
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.67cd4fa4369445b0bb6ea71adf702ed2
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms11071744