Back to Search Start Over

Copy number variant hotspots in Han Taiwanese population induced pluripotent stem cell lines - lessons from establishing the Taiwan human disease iPSC Consortium Bank

Authors :
Ching-Ying Huang
Ling-Hui Li
Wan-Tseng Hsu
Yu-Che Cheng
Martin W. Nicholson
Chun-Lin Liu
Chien-Yu Ting
Hui-Wen Ko
Shih-Han Syu
Cheng-Hao Wen
Zhuge Yan
Hsiang-Po Huang
Hong-Lin Su
Po-Min Chiang
Chia-Ning Shen
Hsin-Fu Chen
B. Lin Ju Yen
Huai-En Lu
Shiaw-Min Hwang
Shih-Hwa Chiou
Hong-Nerng Ho
Jer-Yuarn Wu
Timothy J. Kamp
Joseph C. Wu
Patrick C. H. Hsieh
Source :
Journal of Biomedical Science, Vol 27, Iss 1, Pp 1-15 (2020)
Publication Year :
2020
Publisher :
BMC, 2020.

Abstract

Abstract Background The Taiwan Human Disease iPSC Service Consortium was established to accelerate Taiwan’s growing stem cell research initiatives and provide a platform for researchers interested in utilizing induced pluripotent stem cell (iPSC) technology. The consortium has generated and characterized 83 iPSC lines: 11 normal and 72 disease iPSC lines covering 21 different diseases, several of which are of high incidence in Taiwan. Whether there are any reprogramming-induced recurrent copy number variant (CNV) hotspots in iPSCs is still largely unknown. Methods We performed genome-wide copy number variant screening of 83 Han Taiwanese iPSC lines and compared them with 1093 control subjects using an Affymetrix genome-wide human SNP array. Results In the iPSCs, we identified ten specific CNV loci and seven “polymorphic” CNV regions that are associated with the reprogramming process. Additionally, we established several differentiation protocols for our iPSC lines. We demonstrated that our iPSC-derived cardiomyocytes respond to pharmacological agents and were successfully engrafted into the mouse myocardium demonstrating their potential application in cell therapy. Conclusions The CNV hotspots induced by cell reprogramming have successfully been identified in the current study. This finding may be used as a reference index for evaluating iPSC quality for future clinical applications. Our aim was to establish a national iPSC resource center generating iPSCs, made available to researchers, to benefit the stem cell community in Taiwan and throughout the world.

Details

Language :
English
ISSN :
14230127
Volume :
27
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Biomedical Science
Publication Type :
Academic Journal
Accession number :
edsdoj.67dc5bc3d844d98e58ec1317f016bd
Document Type :
article
Full Text :
https://doi.org/10.1186/s12929-020-00682-7