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Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Authors :
Greta Bigelyte
Joshua K. Young
Tautvydas Karvelis
Karolina Budre
Rimante Zedaveinyte
Vesna Djukanovic
Elizabeth Van Ginkel
Sushmitha Paulraj
Stephen Gasior
Spencer Jones
Lanie Feigenbutz
Grace St. Clair
Pierluigi Barone
Jennifer Bohn
Ananta Acharya
Gina Zastrow-Hayes
Selgar Henkel-Heinecke
Arunas Silanskas
Ralf Seidel
Virginijus Siksnys
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.688a63ea2c9f45e084a40d762048f444
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-26469-4