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Strigolactones Negatively Regulate Tobacco Mosaic Virus Resistance in Nicotiana benthamiana

Authors :
Renyan Huang
Shuaijun Bie
Shan Li
Bin Yuan
Li Zhang
Zhuo Zhang
Jianbin Chen
Weimin Ning
Jing Peng
Yu Zhang
Songbai Zhang
Yong Liu
Deyong Zhang
Source :
International Journal of Molecular Sciences, Vol 25, Iss 15, p 8518 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens but there are currently no reports on their role in the interaction between Nicotiana benthamiana and the tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, a SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. The KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL–TMV–N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.

Details

Language :
English
ISSN :
14220067 and 16616596
Volume :
25
Issue :
15
Database :
Directory of Open Access Journals
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.69447e48c50a4a40ad3d86310e6da5eb
Document Type :
article
Full Text :
https://doi.org/10.3390/ijms25158518